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Investigators noted that ACR20 response was statistically greater than placebo as early as 1 week after the first dose and was sustained through 24 weeks

Amino acid losses during hemodialysis with polyacrylonitrile membranes: effect of intradialytic amino acid supplementation on plasma amino acid concentrations and nutritional variables in nondiabetic patients

10.1074/jbc.M111.335174 J

Methods 10, 2834

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Collect the cells by centrifugation and aspirate the supernatant to the best of your ability, leaving the cell precipitate for backup