Protein concentration was determined using a BCA Reducing Reagent compatible assay kit (catalog: 23250, Thermo Fisher Scientific)
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Kosiborod M, Lam CSP, Kohsaka S, et al
Positive Indicators to Watch For: Deeper, More Restorative Sleep: Often the very first sign, appearing within 1-2 weeks
Consequently, we opted for an integrative cryo-EM AlphaFold-based reconstruction of ice-embedded nozzles, using the two identified proteins, PilQ and NIES39_A07680, as templates (Fig