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After washing, 50 L/well of HRP-conjugated detection antibody (anti-IgM-HRP Sigma A8786) was added for 1 h at RT

measurable glutathione increase within 1-2 weeks

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If you are unfamiliar with the process, our peptide reconstitution guide walks through each step with visual instructions

The dried samples were mixed with 500L of phosphate buffer prepared in D 2 O (pH 7.40 0.05), containing 3-trimethylsilyl-[2, 2, 3, 3, - 2 H 4 ]-propionate (TSP) at a final concentration of 0.33 mM, and then transferred to 5 mm NMR tubes