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Oligo Sequences Codon-optimized SLC25A39 sequence ATGGCAGACCAGGACCCCGCGGGCATCTCACCTCTCCAGCAGATGGTCGCATCTGGAACAGGGG CAGTCGTCACAAGTTTGTTCATGACCCCACTTGATGTAGTGAAAGTCCGGCTTCAATCACAACGCC CTAGCATGGCCAGCGAGCTGATGCCGAGCTCCAGGCTCTGGTCACTTTCTTATACGAAGCTTCCC TCTTCTCTCCAGTCTACGGGTAAATGTTTGCTTTATTGTAACGGCGTACTCGAACCTCTGTATTTGT GTCCAAATGGAGCACGCTGCGCCACGTGGTTTCAGGACCCAACTCGATTTACCGGCACAATGGAC GCATTTGTCAAGATAGTAAGACACGAGGGTACAAGAACGCTTTGGAGCGGCCTCCCTGCTACGTT GGTGATGACGGTTCCCGCAACGGCCATATACTTTACAGCCTACGACCAGCTGAAGGCCTTTCTGT GTGGTAGGGCACTTACCTCAGACCTTTACGCTCCAATGGTCGCAGGGGCCCTTGCAAGACTTGGT ACGGTCACTGTAATAAGTCCGCTCGAACTCATGAGGACAAAACTCCAAGCTCAGCACGTGAGCTA CCGGGAACTGGGGGCTTGTGTACGCACAGCGGTCGCGCAAGGCGGCTGGAGGAGTCTGTGGCT GGGTTGGGGGCCCACGGCCCTCCGGGACGTACCGTTTTCTGCGCTTTATTGGTTTAACTACGAGC TTGTGAAATCTTGGCTCAATGGATTCCGGCCGAAAGACCAGACCTCCGTTGGAATGTCTTTCGTCG CCGGGGGCATTTCCGGCACGGTGGCCGCCGTGCTGACCTTGCCATTCGACGTTGTTAAGACCCA GCGACAGGTCGCTTTGGGGGCAATGGAGGCCGTGCGGGTGAACCCACTCCACGTTGACAGTACA TGGTTGCTGCTCCGCCGCATCCGGGCCGAAAGCGGAACTAAAGGTCTGTTTGCTGGATTTCTTCC GCGAATCATTAAGGCTGCGCCATCTTGTGCAATCATGATCTCTACATACGAGTTTGGAAAATCCTT CTTTCAGAGGCTTAATCAGGACAGACTGCTCGGAGGGTAA SLC25A39 D226A ATGGCAGACCAGGACCCCGCGGGCATCTCACCTCTCCAGCAGATGGTCGCATCTGGAACAGGGG CAGTCGTCACAAGTTTGTTCATGACCCCACTTGATGTAGTGAAAGTCCGGCTTCAATCACAACGCC CTAGCATGGCCAGCGAGCTGATGCCGAGCTCCAGGCTCTGGTCACTTTCTTATACGAAGCTTCCC TCTTCTCTCCAGTCTACGGGTAAATGTTTGCTTTATTGTAACGGCGTACTCGAACCTCTGTATTTGT GTCCAAATGGAGCACGCTGCGCCACGTGGTTTCAGGACCCAACTCGATTTACCGGCACAATGGAC GCATTTGTCAAGATAGTAAGACACGAGGGTACAAGAACGCTTTGGAGCGGCCTCCCTGCTACGTT GGTGATGACGGTTCCCGCAACGGCCATATACTTTACAGCCTACGACCAGCTGAAGGCCTTTCTGT GTGGTAGGGCACTTACCTCAGACCTTTACGCTCCAATGGTCGCAGGGGCCCTTGCAAGACTTGGT ACGGTCACTGTAATAAGTCCGCTCGAACTCATGAGGACAAAACTCCAAGCTCAGCACGTGAGCTA CCGGGAACTGGGGGCTTGTGTACGCACAGCGGTCGCGCAAGGCGGCTGGAGGAGTCTGTGGCT GGGTTGGGGGCCCACGGCCCTCCGGGCAGTACCGTTTTCTGCGCTTTATTGGTTTAACTACGAGC TTGTGAAATCTTGGCTCAATGGATTCCGGCCGAAAGACCAGACCTCCGTTGGAATGTCTTTCGTCG CCGGGGGCATTTCCGGCACGGTGGCCGCCGTGCTGACCTTGCCATTCGACGTTGTTAAGACCCA GCGACAGGTCGCTTTGGGGGCAATGGAGGCCGTGCGGGTGAACCCACTCCACGTTGACAGTACA TGGTTGCTGCTCCGCCGCATCCGGGCCGAAAGCGGAACTAAAGGTCTGTTTGCTGGATTTCTTCC GCGAATCATTAAGGCTGCGCCATCTTGTGCAATCATGATCTCTACATACGAGTTTGGAAAATCCTT CTTTCAGAGGCTTAATCAGGACAGACTGCTCGGAGGGTAA SLC25A39 K329A gccaccATGGCAGACCAGGACCCCGCGGGCATCTCACCTCTCCAGCAGATGGTCGCATCTGGAACA GGGGCAGTCGTCACAAGTTTGTTCATGACCCCACTTGATGTAGTGAAAGTCCGGCTTCAATCACAA CGCCCTAGCATGGCCAGCGAGCTGATGCCGAGCTCCAGGCTCTGGTCACTTTCTTATACGAAGCT TCCCTCTTCTCTCCAGTCTACGGGTAAATGTTTGCTTTATTGTAACGGCGTACTCGAACCTCTGTAT TTGTGTCCAAATGGAGCACGCTGCGCCACGTGGTTTCAGGACCCAACTCGATTTACCGGCACAAT GGACGCATTTGTCAAGATAGTAAGACACGAGGGTACAAGAACGCTTTGGAGCGGCCTCCCTGCTA CGTTGGTGATGACGGTTCCCGCAACGGCCATATACTTTACAGCCTACGACCAGCTGAAGGCCTTT CTGTGTGGTAGGGCACTTACCTCAGACCTTTACGCTCCAATGGTCGCAGGGGCCCTTGCAAGACT TGGTACGGTCACTGTAATAAGTCCGCTCGAACTCATGAGGACAAAACTCCAAGCTCAGCACGTGA GCTACCGGGAACTGGGGGCTTGTGTACGCACAGCGGTCGCGCAAGGCGGCTGGAGGAGTCTGT GGCTGGGTTGGGGGCCCACGGCCCTCCGGGACGTACCGTTTTCTGCGCTTTATTGGTTTAACTAC GAGCTTGTGAAATCTTGGCTCAATGGATTCCGGCCGAAAGACCAGACCTCCGTTGGAATGTCTTTC GTCGCCGGGGGCATTTCCGGCACGGTGGCCGCCGTGCTGACCTTGCCATTCGACGTTGTTAAGA CCCAGCGACAGGTCGCTTTGGGGGCAATGGAGGCCGTGCGGGTGAACCCACTCCACGTTGACAG TACATGGTTGCTGCTCCGCCGCATCCGGGCCGAAAGCGGAACTAAAGGTCTGTTTGCTGGATTTC TTCCGCGAATCATTGCAGCTGCGCCATCTTGTGCAATCATGATCTCTACATACGAGTTTGGAAAAT CCTTCTTTCAGAGGCTTAATCAGGACAGACTGCTCGGAGGGTAA These gene fragments were purchased from Twist Biosciences, and directly cloned into linearized pMXS-IRES-Blast (Cell Biolabs, RTV-016)