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Despite these advantages, the technology faces challenges such as high costs, limited yields, and the absence of bioactive and bio-functional enzymes necessary to produce stable and functional collagen [33, 34]

Monitoring: IGF-1 is often monitored periodically in research settings to track downstream GH-axis response and maintain consistent protocol documentation

Some users adopt a 3-month on, 1-month off strategy to prevent desensitization and maintain receptor sensitivity