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Magnetic beads were washed four times with PBS/BSA and finally resuspended in DMEM/F12 (Invitrogen, Carlsbad, CA, #11320033) medium containing 20% FBS, endothelial cell growth factor (Sciencell, Faraday Ave, Carlsbad, #1052), 100 units/mL penicillin, and streptomycin and plated on gelatin-coated 6-well plates

doi: 10.1002/1097-0215(20000901)87:5 3.0.CO;2-G 63 PandaHRowlandNGKrallCMBowmanBMMajorMBZolkindP

In addition, patients on tirzepatide in the SUMMIT trial experienced improved symptoms and a reduced risk of cardiovascular death or worsening heart failure