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Furthermore, the absence of blood flow and the resulting shear stress altered the properties of endothelial cells in transwell models (Cucullo et al., The first BBB model to include potential effects of shear stress and 3D structure was based on an artificial capillary formed by a polycarbonate fiber pre-coated with fibronectin, rat collagen, or poly-D-lysine

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That single fractionation study is the proof point researchers still cite more than fifty years later as the origin of the entire copper-peptide research field: a compound identified almost by accident in a liver-aging comparison went on to become one of the most studied metallopeptides in dermal and regenerative research

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