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Skeletal muscles and the heart are both major energy consumers

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The reduction in the flavoprotein MTHFR activity observed in individuals with the variant MTHFR 677TT genotype leads to an increase in plasma homocysteine (14)

Quantitative real-time PCR (qRT-PCR) and Western blot assay Total RNA was extracted using Trizol reagent (Invitrogen) according to the manufacturers instructions protocol

Shaw, 2018)