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Scaling production to meet such exponential growth is not as straightforward as with small-molecule drugs
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Downstream functional readouts in myoblast differentiation: IGF-1 LR3 (10 nM, differentiation medium, 5 days) increases myotube diameter 2834% versus vehicle, myotube MyHC (myosin heavy chain) content 3442% by western blot, and fusion index (nuclei within MyHC+ cells / total nuclei) from 46% to 61%
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34 These effects are achieved by the expression and translocation of apical glucose transporter type 4 (GLUT4) thus increasing glucose uptake and by the inhibition of pro-apoptotic proteins such as Bad, Bax and FOXO family proteins, thereby improving beta cell survival
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