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Patients with decreased GSH and increased oxidative stress also showed increased neutrophil influx and IL-8 levels (101)

Western blot analysis involved preparing whole cell lysates using RIPA lysis buffer, quantifying protein concentration via BCA assay, followed by SDS-PAGE, transfer to nitrocellulose membranes, incubation with primary and HRP-conjugated secondary antibodies, and detection using an ECL solution [1]

doi: 10.1016/j.ecoenv.2021.112947 150 YuanATStillmanMJ

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Your body will always crave more glutathione

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