Results Local disruption of Gs signaling in the dorsal vagal complex impairs energy balance and semaglutide-induced weight loss To test whether semaglutide recruits the Gs pathway in hindbrain neurons to drive weight loss, we delivered a virus encoding Cre recombinase and a red fluorescent protein (AAV1-hsyn-cre-P2A-tdTomato) to the dorsal vagal complex (DVC) of Gnas conditional knockout mice 29 (Gnas fl/fl ) to selectively knockout the gene encoding the Gs protein (Fig
However, it is important to note that a direct association between these hormonal changes and weight regain remains inconclusive, as some studies have reported an uncoupling effect where hormone fluctuations do not consistently align with changes in appetite or food intake [12,13,14]
Clinical Chemistry And Laboratory Medicine
The observation was further confirmed by flow cytometry (Fig
This issue becomes even more concerning when the few remaining nephrons become hyperfunctional, further increasing mitochondrial oxidative phosphorylation and ROS production [187,189,190]
The results from our data suggest that initial fibrotic areas likely developed in the model initiation period and did not respond to semaglutide
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