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Furthermore, measurements are susceptible to interference from pH fluctuations, temperature variations, and solvent effects, compromising reliability for absolute quantification ( 3.2.5 Chemiluminescence assay This assay offers exceptional sensitivity for ROS detection through chemiluminescent probes such as luminol and lucigenin, enabling quantification of antioxidant activity via light emission quenching

doi: 10.1056/NEJM199811053391902

Combining them in one preparation is what makes the blend a convenient reference system for in vitro work that needs both activities in a single run

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