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FIGURE 4 Validation of RNA-seq results and the expression analysis of Wnt/-catenin signaling pathway-related genes To verify the results of RNA-seq, 13 differentially expressed genes, including 6 downregulated and 7 upregulated genes, were randomly selected for qRT-PCR assay

Carnitine O -acetyltransferase Carnitine O -acetyltransferase (CrAT), located primarily in the mitochondrial matrix, catalyzes the addition or the removal of carnitine from medium- and short-chain acyl-CoA 60,61 , facilitating the efflux of mitochondrial acetyl-CoA to the cytosol and buffering the intracellular pools of acetyl-CoA and carnitine

Response: Sharpe, Goldsmith and Chalder fail to restore confidence in the PACE trial findings

[10] This low-dose approach contrasted with earlier trials that used much higher doses (e.g., 138 mg or 228 mg daily), which did not significantly outperform placebo