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HFD impairs liver glutathione biosynthesis, vitamin D metabolism genes and genes associated with non-alcoholic fatty liver disease (NAFLD) Genes involved in the GSH biosynthesis pathway were significantly downregulated in the livers of mice fed an HFD compared to those of mice fed a healthy diet (controls) (Fig

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To confirm enzymatic deacetylation by sirtuins, 50 mM nicotinamide was added

NADH levels can be increased in mitochondria by malate-aspartate and the glycerol-3-phosphate redox shuttles 14