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Example-2 Process for the preparation of Semaglutide by using hybrid approach [four fragment approach] Stage- 1 Synthesis of Semaglutide by solution phase peptide fragment condensation: Step-A: Gln(Trt)-Ala-Ala-Lys(C 18diacidmono-t-butyl-y-Glu(AFFA-AFFA)-OtBu)-Glu(OtBu) -Phe-Ile-Ala-Trp(Boc)-Leu-Val-Arg(Pbf)-Gly-Arg(Pbf)-Gly-OtBu (Fragment-6) obtained from stage-3 of example- 1 was dissolved in DMF and stirred for 10 minutes at 25-30 C

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In this sense, the crystallographic structure of KRAS G13C covalently bound to inhibitor 7a was retrieved from PDB under ID 7ok3, and used as a basis for the following steps of the study

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