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Optical density was measured at 450 nm with correction at 540570 nm, and GDF15 concentrations were calculated using a four-parameter logistic (4-PL) standard curve.Each sample was analyzed in duplicate due to limited serum volume and based on the manufacturers standard protocol

Shifts on gut microbiota associated to mediterranean diet adherence and specific dietary intakes on general adult population

These pathways have simultaneously developed for organ-specific adaptation to meet the physiological demands specific to each organ, sensing glucose in pancreatic -cells for insulin secretion, and sensing various nutrients like amino acids, fatty acids, and glucose for GLP-1 secretion in intestinal L-cells 32