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Dulbeccos Modified Eagles Medium (DMEM), fetal bovine serum (FBS), 0.25% trypsin, and penicillin-streptomycin (100) were purchased from Gibco (Grand Island, USA)

However, increased levels have also been reported (68, 72, 73), raising speculation that elevated plasma DPP-4 activity might constitute a risk factor associated with the deterioration of glycaemic control

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It can directly confirm whether the known protein interacts with the detected protein but cant know the binding situation in vivo