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(4) At 24 hours post-transduction, dissociate the transduced cells with Trypsin and replate them at a low density to facilitate optimal growth and selection

Altogether, these results show that C

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Importantly, the failure to reach exact adaptation was not due to the enhanced neural activity since eat16 mutants also showed enhanced responses to a sigmoidal gradient (Fig 3E) and yet, reached exact adaptation following an onstep stimulation (Appendix Fig S4)

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