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(By the way, this is precisely what makes HILIC an orthogonal technique to RPLC!) By contrast, retention in many HILIC columns depends on the semi-immobilized water layer formed at the interface of the polar stationary phase and the organic-rich mobile phase

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10.1038/s41598-024-55008-6 [DOI] [PMC free article] [PubMed] [Google Scholar] 436.Zheng, L

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