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We further confirmed this by measuring GLP-1 levels in the portal blood 1 h after the gavage of fasted mice

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If fewer than 5 days have passed since your scheduled injection, take your normal dose immediately and adjust your schedule forward

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After washing twice with 40 L/well flow buffer, the cells were incubated in flow buffer with 1 nM biotinylated human PD-L1 (ACRO Biosystems) for 30 min at 4 C