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[cited 2024 Apr 12]

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Moreover, using the ubiquitin-rhodamine G (Ub-RhoG) cleavage assay, we observed that purified recombinant USP2 K447R and USP2 C276A both showed drastically lower fluorescence intensity (indicating negligible catalytic activity) compared to WT USP2, with no significant difference in fluorescence intensity between the two mutants at any time point (Fig

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