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High levels of the transcription factor NRF2 can induce metabolic reprogramming in dormant tumor cells, re-establishing redox homeostasis and de novo synthesis of nucleotides, accelerating the activation of tumor cells and tumor recurrence

Real-time PCR was performed using the QuantiTect SYBR Green PCR Kit (TaKaRa Bio, Dalian, China) and an ABI 7500 system (ThermoFisher, Carlsbad, CA, United States)

Enzyme-linked immunosorbent assay of ncAA modified nanobody The recombinant hPD-L1 were diluted in coating buffer (50 mM carbonate buffer, pH 9.6) to a concentration of 20 g/mL