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Downstream functional readouts in myoblast differentiation: IGF-1 LR3 (10 nM, differentiation medium, 5 days) increases myotube diameter 2834% versus vehicle, myotube MyHC (myosin heavy chain) content 3442% by western blot, and fusion index (nuclei within MyHC+ cells / total nuclei) from 46% to 61%

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