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Unfortunately the SFI could not be calculated by the research team as there was poor toe to toe print separation
The first part of the study began by randomly dividing the test subjects in four groups including: wild type, APP/PS1, APP/PS1 + Dihexa administered at a dose of 1.44 mg/kg, and APP/PS1 + Dihexa administered at a dose of 2.88 mg/kg [1]
Cells were fixed with 4% PFA for 30 minutes, permeabilized with 0.1% Triton X-100, and blocked with 5% normal goat or donkey serum
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Total RNA was extracted using TRIzol reagent and quantified with NanoDrop 2000
