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Aim the needle at the inner wall of the vial, not directly onto the lyophilizate powder

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Freezing without a cryoprotectant like glycerol is acceptable for GHK-Cu if single-thaw use is guaranteed, but some researchers add 510% glycerol to the bacteriostatic water during reconstitution specifically to support freeze storage

doi: 10.1016/j.fertnstert.2009.01.114 [DOI] [PubMed] [Google Scholar] 115

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