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If other acids are used, neutralize to pH 7 with triethanolamine, etc., or dilute to an acid concentration of 1% or less before measuring

In addition to its role as a cellular antioxidant, because it is present in such high amounts, glutathione also functions as a thiol buffer, maintaining the sulfhydryl groups of many cellular proteins in their reduced form

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Both methylglyoxal (Figure 3(b)) and CoCl 2 (Figure 3(c)) treatment significantly altered the level of intracellular GSH compared to control (Figure 3(a))

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