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And speaking of shifts, if your body suddenly needs more recovery time than it did in your 30s, its absolutely not a personal failing

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However, certain symptoms may be signs of a more serious complication

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The levels of telomeric DNA in samples were quantified using qPCR, the reference gene used was the single-copy gene 36B4, with primers for both telomeric and 36B4 sequences outlined in Table 1

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