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If the HCD fragmentation had the fragment ion-184.0733 m/z (phosphocholine head group), then the same ions were subjected either to ddMS2 CID (fixed collision energy-32%, activation time-10 ms & Q-0.25, 30K resolution, 100 % normalized AGC target and maxIT-54 ms) or CID MS3 scans (fixed collision energy-35%, activation Q-0.25) triggered on the top 3 most intense ions that lost neutral fatty acids plus ammonia (only for triacylglycerols)

Primary structure of human insulin-like growth factor II

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doi:10.1093/humupd/dmy009