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A membrane-focused variant, membrane-to-protein PRE (mPRE), uses bilayers doped with acyl-chain nitroxide-tagged lipids 9 or lipid headgroup-chelated Gd complexes 29,30 to probe proteinmembrane proximity in both stably bound peripheral proteins and integral membrane proteins 31,32

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It can result in organ malfunction and eventually death [1]

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The key comparison was between the KC group (KD with biotin) and the KBD group (KD without biotin), which allowed the researchers to assess whether KD alone increases biotin requirements or accelerates the development of biotin deficiency

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