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"Effects of DSIP in man." European Neurology

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Blood (10 mL) was collected from the participants in a serum tube without anticoagulant and left for 30 min at room temperature to confirm coagulation, followed by centrifugation at 2,000 g for 10 min

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# 304227, 1:200) in BD Horizon TM Brilliant Stain buffer for 30 min at RT, in the presence of Human TruStain FcX (BioLegend, Cat