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The relative copy numbers of mitochondrial to nuclear DNA were determined by real-time PCR with primers specific to cytochrome c oxidase subunit II (COX II) (mitochondrial) 5-ATCCCAGGCCGACTAAATCA (forward) and 5-TTTCAGAGCATTGGCCATAGAA (reverse) and -actin (nuclear) genes 5-TATCCACCTTCCAGCAGATGT (forward) and 5-AGCTCAGTAACAGTCCGCCTA (reverse)