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Theor Biol Med Model 2012;9:55

PMID: 21136578

To inhibit mitochondrial metabolism and GSH synthesis, the following chemicals were purchased from Sigma: metformin, rotenone, antimycin A, oligomycin A and BSO

Read the absorbance at 405 nm or 415 nm using a microplate reader

For example, the combination of the cancer drug Dasatinib and the natural compound Quercetin was shown to induce apoptosis in senescent cells in a variety of contexts in vitro and in vivo 1,4

Specifically, proteins associated with DNA damage, inflammation, and oxidative stress, including Cop1, H2-D1, Ndufa6, and Fam76a, showed downregulation following D-CuP treatment