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Viral DNA was extracted from blood samples of both captive and free-ranging khulan using a commercially available kit (QIAamp DNA Blood Mini Kit, Giagen) and from nasal swabs using the Invisorb Spin Virus DNA Mini Kit, Stratec, according to the manufactures instructions
Although self-esteem scores in the SAD group were within the normal range, they may have mitigated the worsening of symptomatology because self-esteem acts as a mediating element in stressful situations
Activation of POMC neurons promotes satiety, while inhibition of NPY neurons reduces hunger signaling
Ann Intern Med 1998
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