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The staining intensity was assessed in five randomly selected sample fields from each group by an expert pathologist who was blinded to the experimental groups, and the results are presented as a percentage of positive expression in a total of 500 neurons 64

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That being said, an alternative measure was used by the research team that assessed motor function by grading the toe and heel foot print characteristic on a scale from 0-4, with 0 defined as no print, non functional, and 4 defined as complete print, near normal functioning [2]

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