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Use PBS where sensitivity is high, and saline for most robust peptides

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Without prebiotic fiber, without polyphenol-rich foods, without adequate dietary diversity, the probiotic strains cannot thrive or produce the metabolites that drive GLP-1 production

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The second antibody was a biotinylated goat anti-rabbit immunoglobulin, and the signal was detected using the Vectastain ABC kit (Vector Laboratories, Burlingame, California, USA) with DAB as a substrate