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Journal of Clinical & Medical Surgery, 3 (2), 1127

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After centrifugation, methanol-containing proteins were precipitated with cold trichloroacetic acid (TCA) and reduced with 500 mM sodium borohydride in 0.2 M borate buffer (pH 9.2) after overnight incubation at room temperature

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Its useful in short-term therapeutic windows but less ideal for long-term use due to cost, invasiveness and the need for clinical oversight, he said