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Computational and mutational analysis of human glutaredoxin (thioltransferase): probing the molecular basis of the low pKa of cysteine 22 and its role in catalysis

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The sample size was determined using SPSS 25.0 software (SPSS, Inc., Chicago, IL, USA) to achieve 80% study power, a type one error of 5%, and a 95% statistical significance (P = 0.05)

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