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As expected, when increasing the incubation time with GSH, UPFB displayed a time-dependent GSH consumption capacity (Figure 7E)

Materials and methods Reagents and materials Oxidized glutathione (GSH) 2 , homocystine (Hcy) 2 , cystine (Cys) 2 , TCEP, benzyl bromide, 2-chloropyridine, L-lysine, N-acetylcysteine (NAC), 2-mercaptoethanesulfonic acid (MESNA), N-(2-mercaptopropionyl)-glycine (MPG), [2 S]-1-[3-mercapto-2-methylpropionyl]-L-proline (Captopril), and lipoic acid (LA), and ethylenediaminetetraacetic acid (EDTA) were received from the Sigma Aldrich Company (St

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Menge BA, Schrader H, Breuer TG et al (2009) Metabolic consequences of a 50% partial pancreatectomy in humans