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Cells were mixed with transfection reagents and plated at 13,000 cells per well into 96-well Greiner CELLSTAR white-walled plates (Sigma-Aldrich), and assays were performed 48 h later

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4) Should symptoms occur, suspend use and consult a medical professional

Moreover, the levels of MDA and LDH were increased, and the Nrf2/HO-1/HMGB1 pathway was inhibited, supporting the hypothesis of oxidative stress injury in ferroptosis

The principle is exactly the same

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