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In the Wnt pathway, the key step of the Wnt pathway is the binding of the Wnt protein to the Frizzled receptor, and the nuclear redox protein (NRX) blocks Disheveled (DVL) and plays a negative regulatory role in the Wnt pathway (Yu et al., 2021)
Assays were initiated by replacing growth medium with 650 l of low-buffered RPMI-1640 growth medium without sodium bicarbonate, HEPES, and containing only 1% FBS
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