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Anti-FNDC5 (ab174833) and anti-ATGL were from Abcam, while anti-UCP-1 and anti--actin antibodies were purchased from Sigma-Aldrich

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Elsevier, Amsterdam, pp 429439 Nelen WLDM, Blom HJ, Thomas CMG, Steegers EAP, Boers GHJ, Eskes TKAB (1998) Methylenetetrahydrofolate reductase polymorphism affects the change in homocysteine and folate concentrations resulting from low dose folic acid supplementation in women with unexplained recurrent miscarriages

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3a) in addition to superfusing it with extracellular fluid

Higher starting weight often leads to faster early results