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These cell lines have defects affecting the enzymes involved in the respiratory chain and are, therefore, unable to generate an electrochemical gradient across the mitochondrial membrane, concomitant with the loss of the glucose-induced increase of intracellular Ca 2+ [1113]

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With one set of 20 strokes and another set of 10 strokes, the remaining sample was homogenized using a 2-ml homogenizer

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