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Single particle tracking (SPT) of human GLP-1R fused to monoclonal EGFP (hGLP-1R-mEGFP) WT versus V229A imaged by total internal reflection fluorescence (TIRF) microscopy also showed reduced total displacement and speed (displacement over time) of V229A versus WT receptors under vehicle conditions, with these parameters also decreased following WT GLP-1R stimulation with exendin-4 (Figure 4C)