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Neutrophils were pelleted at 250 g for 6 min, washed in RPMI 1640 with 2% FBS, 2 mM L-glutamine and 10 mM HEPES, and centrifuged at 250 g for 4 min

Pinealon and other neurological peptides warrant consideration for chronic nerve pain, though evidence remains more limited compared to tissue-healing peptides

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After this time, the unused portion should be discarded