The hCTR cells were cultured in growth medium consisting of Dulbeccos Modified Eagles Medium (DMEM, Gibco, #31966-021) supplemented with 10% v/v foetal bovine serum (FBS, Gibco, #16140-071), 1% v/v Penicillin-Streptomycin (Gibco, #15140-122), 0.6 mg/mL Geneticin G418 (Gibco, #10131-027) and 0.2 mg/mL Hygromycin (Invitrogen, #10687010)
These variants may help provide context about your baseline glutathione synthetic capacity and phase II detoxification enzyme function, supporting a more informed conversation with your provider about whether supplementation aligns with your genetic predispositions
Targeting MET in cancer: rationale and progress
However, the physical "cost" of building a placenta and supporting fetal development can sometimes stretch these resources thin
Trivedi MS, et al
Not sure whether this was due to improper storage - it needs to be kept cool and in the dark otherwise can degrade - or for some other reason