Cultures were pulse-labeled with 10 M BrdU (a nucleoside analog that competes with thymidine for incorporation into DNA) in DMEM-10% HS for the final 2-h period of 48-h in culture, followed by immunocytochemistry for detection of BrdU using G3G4 anti-BrdU mAb (1:100 dilution) and HRP-conjugated anti-mouse IgG Ab (1:500 dilution)
In terms of mechanisms of action, studies have shown that there is no change in the plasma concentration of GSH, except locally, with more pronounced effects on the upper and lower respiratory tract
Think of glutathione as your body's cellular superhero - always on duty, never taking a break
Cell 2016
The antioxidant role of GSH GSH is implicated in several functions, including antioxidant defense with reduction of oxidative stress and maintenance of redox balance, metabolic detoxification from xenobiotics and exogenous compounds, cell cycle regulation, and immune system modulation, as well as fibrogenesis (1)
10.1161/01.CIR.0000092184.43176.91 Circulation