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DISCUSSION Immune checkpoint inhibition is a novel treatment strategy intended to activate the immune system against malignancy.1 Pembrolizumab is an IgG4 antagonist monoclonal antibody to programmed-cell-death-1 (PD-1) receptor approved by the FDA in 2014.1,2 When PD-1 binds to programmed-cell-death-ligand-1 (PD-L1), T-cell proliferation is suppressed

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Acknowledgements We thank National Laboratory Animal Center (NLAC), NARLabs, Taiwan, for technical support in service of isolator

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