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Storage mistake: if reconstituted DSIP was left at room temperature, treat the vial as compromised

5di) in HFD-fed mice

Both openings could provide access to the catalytic triad, as they have negative electrostatic potential and would attract the positively-charged N-terminus of the short bioactive peptide substrate (Rasmussen et al., 2002)

Other variants showed similar Tm values to those of the wild type