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NIA mice were sufficiently acclimated for 4 months in our vivarium until they were considered middle-aged (12 mo.) and old (22 mo.) at the start of MOTS-c injections

The quantitative analysis of each sample was performed by Exion ultra-high performance liquid chromatography (UPLC) coupled with a triple quadrupole mass spectrometer (SCIEX, QTRAP 6500+), equipped with electrospray IonDrive Turbo-V Source

Prolactin production is now stimulated and maintained by the infant (or the pump) suckling on the breast

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