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The column was washed with 6 bed volumes of wash buffer (50 mM sodium phosphate, 300 mM NaCl and 100 mM imidazole, pH 7.4) and the target protein was eluted with 6 bed volumes of elution buffer (50 mM sodium phosphate, 300 mM NaCl and 300 mM imidazole, pH 7.4)
Eur J Med Genet 1(71):104962 Maruo Y, Nakahara S, Yanagi T, Nomura A, Mimura Y, Matsui K et al (2016) Genotype of UGT1A1 and phenotype correlation between CriglerNajjar syndrome type II and Gilbert syndrome
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In the study above, the authors note that more drugs [from postauricular injection] concentrated in the inner ear for longer therapeutic time, and less systemic delivery implied less risk of side effects. Practical Considerations: Injection Scheduling Protocol typically involves multiple injections over 12 weeks